Microbial Community Responses to Deepwater Horizon Oil Spill: Pre-Spill Background Assessment
Louisiana State University
DWH Project Funding
Known Leveraged Funding
Gulf of Mexico Research Initiative (GoMRI)
Gulf of Mexico Research Initiative GoMRI Grant Program
Human and/or Institutional Capacity
This project leverages a set of samples obtained in March, 2010 prior to the DWH oil spill to determine the composition and structure of bacterial communities in the northern Gulf of Mexico. DNA extracts from bacterioplankton collected along nearshore to off-shore transects and from 2 m to 1700 m depth have been used in PCR amplifications of 16S rRNA genes. Amplification has used a bar-coding protocol to enable high throughput pyrosequencing, resulting in more than 220,000 reads over 44 distinct samples.